England’s leading Test wicket-taker James Anderson will play no further part in the Ashes against Australia after a recurrence of a calf injury.
The 37-year-old Lancashire seamer bowled only four overs in the first Test and missed the second and third.
He felt pain in the calf on Thursday during a four-day friendly for Lancashire second XI.
Somerset’s Craig Overton has been named in England’s 13-man squad for the fourth Test at Old Trafford.
It is also expected that England will shuffle their batting order by swapping opener Jason Roy and number four Joe Denly.
Roy made his Test debut in this series but has struggled for runs, making an average of just 16.75 in four matches, while Denly has a top score of 50 in his three games.
The match – at Anderson’s home ground – starts on 4 September, with the five-Test series level at 1-1.
England won a thrilling third Test at Headingley by one wickets thanks to Ben Stokes’ remarkable unbeaten 135.
England squad for fourth Ashes Test against Australia: Joe Root (capt), Jofra Archer, Jonny Bairstow (wk), Stuart Broad, Rory Burns, Jos Buttler (wk), Sam Curran, Joe Denly, Jack Leach, Craig Overton, Jason Roy, Ben Stokes, Chris Woakes.
Anderson’s absence means England are likely to name an unchanged pace attack of Jofra Archer, Stuart Broad and Chris Woakes.
Anderson suffered a calf injury in a County Championship match against Durham in June but was passed fit for the opening Ashes Test.
However, he left the field on the first morning and did not bowl in the remainder of the game, which England lost.
Anderson took 2-23 from nine overs for Lancashire second XI in a friendly against Leicestershire on 20 August.
He bowled 20 overs against Durham second XI on Tuesday but, after a nine-over spell on Thursday, he was reviewed by the England and Wales Cricket Board medical staff and ruled out of the Ashes.
Seamer Overton, 25, has taken seven wickets at an average of 42.28 in three Tests since making his debut in the 2017-18 Ashes in Australia. His most recent appearance was the defeat by New Zealand in Auckland in March 2018.
He has 32 wickets at 21.34 apiece in eight County Championship matches this season.
Opening batsman Jason Roy keeps his place despite scoring only 57 runs in six innings against Australia.
Surrey team-mate Ollie Pope, who was added to the squad as concussion cover for Roy before the third Test, has been overlooked.
The final Test at The Oval begins on 12 September.
England must win the series to regain the Ashes, while Australia, as holders of the urn, need only a series draw to retain them.